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Large haematoxylin‐stainable keratohyaline granules in solar keratoses: immunohistochemical comparison using anti‐Ted‐H‐1 antibody and antiloricrin antibody
Author(s) -
Takahashi M.,
Horiuchi Y.,
Tezuka T.
Publication year - 2005
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.2005.06866.x
Subject(s) - haematoxylin , monoclonal antibody , immunoelectron microscopy , immunohistochemistry , antibody , polyclonal antibodies , microbiology and biotechnology , chemistry , staining , antigen , pathology , biology , medicine , immunology
Summary Background Our previous study showed that large keratohyaline granules (KHG) in molluscum contagiosum that stained with haematoxylin also reacted with anti‐Ted‐H‐1 monoclonal antibody (mAb), but not with antifilaggrin mAb or antiloricrin polyclonal antibody (pAb). This finding indicated that the Ted‐H‐1 antigenic protein is a haematoxylin‐stainable protein in KHG. Objectives To clarify the identity of the major component protein of the large KHG in solar keratosis, another disorder in which large KHG are observed. Methods An enzyme immunohistochemical study was performed using antifilaggrin mAb, anti‐Ted‐H‐1 mAb and antiloricrin pAb. Immunofluorescent double staining and immunoelectron microscopic analyses were performed using anti‐Ted‐H‐1 mAb and antiloricrin pAb. Results Antifilaggrin mAb, anti‐Ted‐H‐1 mAb and antiloricrin pAb reacted with normal KHG in nonlesional skin of solar keratosis, while only anti‐Ted‐H‐1 mAb reacted with the large KHG in the lesions of solar keratosis. Antifilaggrin mAb did not react with large KHG. Antiloricrin pAb reacted with the cell membrane of the stratum granulosum, but not with large KHG. Conclusions These findings suggest that the haematoxylin‐stainable protein in the large KHG would be a Ted‐H‐1 antigen protein which was neither filaggrin nor loricrin.