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Expression of intercellular adhesion molecule‐1 in UVA‐irradiated human skin cells in vitro and in vivo
Author(s) -
TREINA G.,
SCALETTA C.,
FOURTANIER A.,
SEITÉ S.,
FRENK E.,
APPLEGATE L. A.
Publication year - 1996
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1996.tb01154.x
Subject(s) - human skin , dermis , in vivo , staining , intercellular adhesion molecule 1 , cell adhesion molecule , in vitro , immunohistochemistry , icam 1 , epidermis (zoology) , microbiology and biotechnology , chemistry , biology , pathology , immunology , biochemistry , medicine , anatomy , genetics
Summary Ultraviolet A (UVA) radiation represents an important oxidative stress to human skin and certain forms of oxidative stress have been shown to modulate intercelluar adhesion molecule‐1 (ICAM‐1) expression. ICAM‐1 has been shown to play an important part in many immune reactions and the perturbations of this molecule by ultraviolet radiation could have implications in many inflammatory responses. An enhancement immunohistochemical method with avidin/biotin was used for analysing the early eflects of UVA radiation on human cell cultures and human skin (340–400nm). Both in vitro in and in vivo show that ICAM‐1 staining in epidermal keratinocytes. which was expressed constitutively. decreased in a UVA dose‐dependent manner. The decrease was most noted at 3–6h following UVA radiation with some ICAM‐1 staining returning by 48h post‐UVA. ICAM‐1 positive staining in the dermis was specific for vascular structures and was increased 24h after UVA radiation. Cultured dermal fibroblasts exhibited ICAM‐1 staining which increased slightly within 6–48h post‐UVA radiation. As epidermal ICAM‐1 expression is depleted following UVA radiation and dermal expression increases due to an increase in the vascular structures. ICAM‐1 provides a valuable marker following UVA radiation in human skin that can be readily measured in situ .