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p53 protein expression in viral warts from patients with epidermodysplasia verruciformis
Author(s) -
PIZARRO A.,
GAMALLO C.,
CASTRKSANA J.S.,
GOMEZ L.,
PALACIOS J.,
BENITO N.,
ESPADA J.,
FONSECA E.,
CONTRERAS F.
Publication year - 1995
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1995.tb08704.x
Subject(s) - epidermodysplasia verruciformis , immunostaining , immunohistochemistry , p53 protein , monoclonal antibody , polymerase chain reaction , exon , virology , biology , virus , medicine , gene , antibody , pathology , immunology , genetics
Summary The p53 protein is the product of a tumour suppressor gene, which is implicated in many human malignancies. p53 expression was investigated by immunohistochemistry in a series of viral warts (n = 12) from five patients with epidermodysplasia verruciformis (EV), using a monoclonal anti‐p53 antibody (D07). p53 expression was also investigated in a series of common warts (n = 8), flat warts (n= 8), and penile bowenoid papulosis (n = 6) from non‐EV patients. Immunostaining was positive in 11 of 12 (92%) EV warts, whereas p53 reactivity was negative in most cases of warts from non‐EV patients. Exons 5–8 of the p53 gene were screened by the polymerase chain reaction‐single strand conformation polymorphism technique in four EV warts, which were strongly stained for p53, and p53 mutations were not detected. These results suggest an association between p53 accumulation (probably of wild type) and EV warts.