Premium
In situ detection of retinoid‐X receptor expression in normal and psoriatic human skin
Author(s) -
REICHRATH J.,
MUNSSINGER T.,
KERBER A.,
ROCHETTEEGLY C.,
CHAMBON P.,
BAHMER F.A.,
BAUM H.P.
Publication year - 1995
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1995.tb02612.x
Subject(s) - retinoid x receptor alpha , retinoid x receptor , receptor , involucrin , biology , retinoid , keratinocyte , keratin , retinoic acid , hair follicle , human skin , retinoic acid receptor alpha , nuclear receptor , endocrinology , psoriasis , retinoid x receptor gamma , medicine , microbiology and biotechnology , retinoic acid receptor , cell culture , immunology , biochemistry , paleontology , gene , transcription factor , genetics
Summary Increasing evidence suggests that the retinmd‐X receptors RXR(‐α,‐β‐γ) play a crucial part in regulating the transcription a I activity of several steroid hormone receptors, including 1.25‐dihydroxyvitamin D 3 receptors (VDR) and retinoic acid receptors (RAR‐α,‐β,‐γ). We developed a new technique for immunohistochemical in situ detection of RXR receptors, and investigated the localization of RXR‐a in normal and psoriatic human skin using a recently raised corresponding specific antibody. RXR‐α positive cells related to the skin were phenotyped by sequential sections and a double‐labelling procedure for the simultaneous demonstration of this nuclear receptor and cell membrane antigens, as well as cytokeratin 10. HLA‐DR and vimentin. Our findings indicate that: (i) RXR‐m is strongly expressed in normal and psoriatic human skin: (ii) most of the cell types in normal human skin, including keratinocytes, melanocytes, fibroblasts andskin immune cells such as Langerhans cells, reveal strong nuclear immunoreactivity for RXR‐a, with less cytoplasmic staining; (Hi) altered levels or distribution of RXR‐n in the skin do not appear to be involved in the genesis of psoriasis vulgaris, but subepidermal and subcellular distribution suggest a function of RXR‐α in the transition from proliferation to differentiation in epidermal keratinocytes: (iv) expression in the hair follicle points to a contribution from RXR‐α to hair growth.