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Aberrant accumulation of p53 associates with Ki67 and mitotic count in benign skin lesions
Author(s) -
SOINI Y.,
KAMEL D.,
PÄÄKKÖ P.,
LEHTO V.P.,
OIKARINEN A.,
VÄHÄKANGAS K.
Publication year - 1994
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1994.tb08552.x
Subject(s) - mitosis , medicine , dermatology , pathology , biology , genetics
Summary Sixty‐two skin samples from patients with a variety of benign disorders (20 cases of psoriasis, 14 cases of chronic dermatitis, 11 seborrhoeic keratoses, 11 cases of lichen planus), and seven normal skin samples, were stained immunohistochemically with a polyclonal antibody (CM‐1) to p53, and a monoclonal antibody to Ki67, using the avidin‐biotin complex method, p53‐positive keratinocytes could be found in most of these lesions. The percentage of p53‐positive cells was, however, far lower than usually seen in p53‐positive malignant tumours. No p53 reactivity was observed in the normal skin samples. Variable Ki67 reactivity was observed in all skin samples. Overall, the number of Ki67‐positive cells was higher in skin samples in which the proportion of p53‐positive cells was high (>0.5% of total epidermal cell population) ( P =0.004). This also applied separately to psoriatic and non‐psoriatic lesions ( P =0.028 and P =0.033, respectively). In cases with >10% of Ki67‐positive cells, there were significantly more mitoses ( P <0.001). This association applied to both psoriasis and the other lesions studied ( P =0.024 and P <0.001, respectively). The results show that immunohistochemically detectable accumulation of p53 is a frequent finding in non‐neoplastic skin lesions. As p53 positivity was associated with the proliferation marker Ki67, the accumulation of p53 is possibly a response to an increased proliferation rate of the keratinocytes in these skin diseases, or alternatively it may be associated with apoptosis.