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Are excessive granulation tissue formation and retarded wound contraction due to decreased collagenase activity in wounds in tight‐skin mice?
Author(s) -
SÅGREN M.,
MERTZ P.M.
Publication year - 1994
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1994.tb08520.x
Subject(s) - granulation tissue , collagenase , contraction (grammar) , wound healing , granulation , pathogenesis , medicine , chemistry , pathology , anatomy , surgery , endocrinology , materials science , biochemistry , enzyme , composite material
Summary Wound contraction is delayed in tight‐skin mice but the mechanism(s) are unknown. The purpose of this study was to investigate collagenase levels and the formation of granulation tissue in experimental wounds in tight‐skin mice. One full‐thickness skin excision (20×20 mm) was made on the back of nine tight‐skin and eight normal mice. Granulation tissue analyses were performed 7 days post‐operatively. The collagenase activity was determined by the use of a radiolabelled telopeptide‐free collagen substrate, and the amount of granulation tissue was determined gravimetrically. Wound contraction was delayed ( P <0.001) in tight‐skin mice (mean 22%) compared with normal mice (mean 46%). The collagenase activity was decreased ( P <0.05) by 40%, whereas the quantity of granulation tissue was increased ( P <0.001) by 60% in the wounds of tight‐skin mice. Decreased collagenase content may provide one explanation for the delayed contraction of full‐ thickness wounds in tight‐skin mice. Furthermore, this animal wound model may prove useful in the understanding of the pathogenesis, and in exploration of treatment, of excessive granulation tissue formation during wound healing.