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A comparative study of the cytotoxicity of skin irritants on cultured human oral and skin keratinocytes
Author(s) -
EUN H.C.,
CHUNG J.H.,
JUNG S.Y.,
CHO K.H.,
KIM K.H.
Publication year - 1994
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1994.tb06877.x
Subject(s) - cytotoxicity , benzalkonium chloride , keratinocyte , neutral red , oral mucosa , human skin , chemistry , staining , cell culture , pathology , microbiology and biotechnology , biology , in vitro , medicine , biochemistry , chromatography , genetics
Summary The cytotoxicity of irritants on human oral and skin keratinocyte culture models was compared. Keratinocytes were exposed to sodium lauryl sulphate and benzalkonium chloride at concentrations of 10 –4 –10 –7 M for 24 h. Cytotoxicity was evaluated by changes in niitochondrial metabolic activity (MIT assay) and plasma membrane integrity (LDH leakage). Our results showed that oral and skin keratinocytes are equally sensitive to the irritants tested. There were marked similarities in susceptibility between each cell type cultured from six individuals. The immunohistochemical staining pattern of both cell types resembled that of the basal cell. These observations suggest that the skin keratinocyte culture model may be useful in evaluating the cytotoxicity of agents which are irritants on either the skin or oral mucosa.