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Quantification of hydrogen peroxide generation by Granuflex™ (DuoDERM™) Hydrocolloid Granules and its constituents (gelatin, sodium carboxymethylcellulose, and pectin)
Author(s) -
SCHMIDT R.J.,
CHUNG L.Y.,
TURNER T.D.
Publication year - 1993
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1993.tb03518.x
Subject(s) - hydrogen peroxide , gelatin , pectin , chemistry , catalase , horseradish peroxidase , nuclear chemistry , sodium , chromatography , biochemistry , organic chemistry , antioxidant , enzyme
Summary The hydrogen peroxide generating capacity of Granuflex™ Hydrocolloid Granules and its constituents (porcine gelatin, sodium carboxymethylcellulose and pectin) was examined using the scopoletinhorseradish peroxidase assay in the presence and absence of catalase. Oxygen purging reduced the formation of hydrogen peroxide by 77–96%. The total concentrations of hydrogen peroxide detected were 1.9 × 10 −6 , 1.2 × 10 −6 and 2.3 × 10 −6 mol/1 for Granuflex. pectin and gelatin (using 0.5% w/v), respectively, after 48 h incubation in a phosphate buffer. pH 7.4, at 37°C, No hydrogen peroxide was formed by sodium carboxymethylcellulose. The results indicate that hydrogen peroxide generation by Granuflex may be ascribed to its gelatin and pectin components, but not to the sodium carboxymethylcellulose. The release of low levels of hydrogen peroxide into the wound environment could conceivably contribute both to the inflammatory phase and to fibroblast proliferation, and hence to the granulation phase of wound healing.