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Modification of neutrophil functions by naftifine
Author(s) -
SOLOMON B.A.,
LEE W.L.,
GEEN S.C.,
SUNTHARALINGAM K.,
FIKRIG S.M.,
SHALITA A.R.
Publication year - 1993
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1993.tb00197.x
Subject(s) - chemotaxis , zymosan , superoxide , stimulation , respiratory burst , in vitro , neutrophile , in vivo , chemistry , chemokinesis , granulocyte , pharmacology , biochemistry , microbiology and biotechnology , immunology , biology , endocrinology , receptor , enzyme
Summary Naftifine (NF), a topical antimycotic agent, is highly active in vitro and in vivo against a wide range of pathogenic fungi. NF inhibits human polymorphonuclear leucocyte (PMN) chemotaxis. Following stimulation with zymosan‐activated serum. 85–97% of the PMNs exhibited detectable membrane ruffling and polarity. In contrast, NF‐treated PMNs did not exhibit such chemotactic factor‐induced shape changes. We also analysed the effect of NF on PMN superoxide anion (O 2 − ) and chemiluminescence (CL) production, as a measure of respiratory burst activity. Stimulation of PMNs pre‐incubated with NF (37°C for 30 min at 1–150 μg/ml) hy FMLP, PMA and zymosan resulted in a dose‐dependent inhibition in PMN CL. Doses of NF which depressed chemotaxis, inhibited CL and diminished O 2 − production in a statistically significant manner ( P <0.05–0.001). In conclusion, NF alters membrane‐related responses in PMNs, and this alteration may be associated with a change in PMN morphology. Binding of NF to PMN membrane sterol, with a subsequent alteration in membrane configuration, is the most likely cause of the inhibition of PMN function. The data collectively document biochemical and morphological differences between control and NF‐treated PMNs as determined by stimulus‐specific CL and O 2 − generation and membrane shape change. Such differences may account, in part, for its efficacy in inflammatory fungal skin diseases.