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Isolation and maintenance of the human pilosebaceous duct: 13‐ cis retinoic acid acts directly on the duct in vitro
Author(s) -
GUY R.,
RIDDEN C.,
BARTH J.,
KEALEY T.
Publication year - 1993
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1993.tb00165.x
Subject(s) - retinoic acid , duct (anatomy) , thymidine , in vivo , biology , in vitro , epithelium , biochemistry , cell culture , endocrinology , anatomy , medicine , genetics
Summary The human pilosebaceous duct was isolated and maintained for 7 days in defined medium, and defined medium supplemented with 1 μm 13‐ cis retinoic acid. Freshly isolated ducts retained their in vivo morphology, showing a stratified squamous keratinizing epithelium. On maintenance there was a loss of basic duct architecture, and a significant reduction in the rate of [methyl‐ 3 H] thymidine uptake. The addition of 1 μM 1 3‐cis retinoic acid resulted in an improved duct architecture and caused a further signiticant reduction in the rate of [methyl‐ 3 H] thymidine uptake. [Methyl‐ 3 H] thymidine autoradiography showed that freshly isolated ducts maintained their in situ pattern of cell division. It was difficult to discern the region of celi division in ducts maintained for 7 days, but the degree of graining reflected the measured rates of [methyl‐ 3 H] thymidine uptake into PCA precipitable material. The pattern of keratin synthesis of the freshly isolated duct was similar to patterns previously described for the duct in situ . This study reports the successful isolation and maintenance of the human pilosebaceous duct, and demonstrates that 13‐cis retinoic acid acts directly at the level of the duct.