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Localization of transglutaminase 1 mRNA in normal and psoriatic epidermis by non‐radioactive in situ hybridization
Author(s) -
OMURA K.,
YAMANISHI K.,
HOSOKAWA Y.,
DOI H.,
HIRANO J.,
FUKUSHIMA S.,
YASUNO H.
Publication year - 1993
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1993.tb00141.x
Subject(s) - library science , medicine , dermatology , computer science
Summary We examined the expression of the transglutaminase 1 (TGase 1) gene IN frozen sections of normal and psoriatic epidermis by means of non‐radioactive in situ hybridization with digoxigenin‐labelied cRNA probes. TGase l mRNA was expressed in the granular layer of normal epidermis. regardless of ortho‐or hyperkeratosis. However, in psoriatic epidermis. TGasel mRNA was detected in the suprabasal spinous layer, but not in the subcorneal layer. These results indicate that TGase 1 gene expression is limited to the last stage of keratinization in normal epidermis and this regulation is disturbed in psoriasis.