Premium
Expression of HLA class II antigens on skin fibroblasts in scleroderma
Author(s) -
BRANCHET M.C.,
BOISNIC S.,
BLÉTRY O.,
ROBERT L.,
CHARRON D.,
FRANCÈS D.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb15112.x
Subject(s) - scleroderma (fungus) , human leukocyte antigen , immunology , antigen , class (philosophy) , medicine , biology , computer science , inoculation , artificial intelligence
Summary Skin biopsies were taken from 11 patients with morphoea, nine with acrosclerosis and 10 with diffuse systemic sclerosis and processed for immunohistochemical studies using a panel of monoclonal antibodies including antibodies to MHC class II antigens. A significantly higher percentage of HLA‐DR positive dermal cells were observed in the reticular dermis in biopsies from patients with morphoea (44·1 ± 16·2%). acrosclerosis (15·9 ± 5·4%) and systemic sclerosis (39·5 ± 2·3%) when compared with the controls (6·6 ± 2%), A smaller percentage of dermal cells also expressed HLA‐DP and ‐DQ. The degree of mononuclear cell infiltrate in the biopsies, however, did not correlate with the percentage of HLA class II positive fibroblasts. In organ culture, the expression of the HLA class II antigens was almost totally lost after 3 days and was no longer detected on fibroblasts after 3 weeks of culture.