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Substance P induces intracellular calcium increase and translocation of protein kinase C in epidermis
Author(s) -
KOIZUMI H.,
TANAKA H.,
FUKAYA T.,
OHKAWARA A.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb14872.x
Subject(s) - epidermis (zoology) , chromosomal translocation , intracellular , calcium in biology , microbiology and biotechnology , calcium , chemistry , protein kinase a , protein kinase c , kinase , biology , biochemistry , medicine , anatomy , gene
Summary Substance P is a neuropeptide present in, and released from, peripheral C nerve endings. The presence of substance P‐positive nerve fibres in the epidermis has been reported. We investigated the effect of substance P on the transmembrane signalling system of pig epidermal keratinocytes. Treatment of pig epidermis with substance P resulted in an increase in inositol 1,4,5‐trisphosphate (IP 3 ), and in intracellular free calcium. The treatment also resulted in translocation of protein kinase C from a cytosol to a membrane fraction. Substance P, however, did not affect the β‐adrenergic‐ or histamine (H2)‐ adenylate cyclase responses of the epidermis. Neither forskolin‐induced, nor cholera toxin‐induced cyclic AMP accumulation were affected by substance P treatment. These results are consistent with the view that substance P stimulates phosphatidylinositol‐4,5‐bisphosphate (PIP 2 ) hydrolysis of keratinocytes, resulting in IP 3 ‐Ca 2+ and diacylglycerol‐protein kinase C signal activation. Although protein kinase C is known to affect the epidermal adenylate cyclase system, no evidence for such ‘cross‐talk regulation’ was detected in keratinocytes by substance P treatment.