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Isolated basal keratinocytes express pemphigoid gestationis antigen
Author(s) -
KELLY SUSAN E.,
McVITTIE EVA,
BLACK M. M.,
FLEMING S.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb08401.x
Subject(s) - trypsinization , bullous pemphigoid , immunofluorescence , antigen , pemphigoid , indirect immunofluorescence , basal (medicine) , trypsin , chemistry , keratinocyte , immunology , medicine , antibody , dermatology , pathology , microbiology and biotechnology , biology , in vitro , endocrinology , biochemistry , insulin , enzyme
Summary Basal keratinocytes were isolated from epidermal cell suspensions prepared by trypsinization of normal human skin. Cells were identified as basal cells by their adherence to collagen and confirmed as basal cells by the presence of pemphigoid antigen. Using an indirect immunofluorescence assay, cells wee found to express pemphigoid gestationis‐related antigen. Sera from patients with pemphigoid gestations reacted in one of two immunofluorescence patterns: either polar, in a pattern similar to that observed with bullous pemphigoid serum, or with uniform staining around the cell periphery. Pemphigoid gestationis‐related antigen is expressed by isolated basal keratinocytes and is resistent to trypsinizatio. The heterogeneity of immunofluorescence patterns may correspond to the heterogeneity of antigen recognition by different patients with pemphigoid gestationis.