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Effects of capsaicin, bradykinin and prostaglandin E 2 in the human skin
Author(s) -
WALLENGREN JOANNA,
HÅKANSON R.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb07806.x
Subject(s) - capsaicin , bradykinin , prostaglandin e2 , human skin , medicine , prostaglandin e , pharmacology , chemistry , endocrinology , biology , receptor , genetics
Summary The actions and interactions of putative mediators of inflammation, such as substance P (SP), histamine, bradykinin and prostaglandins (PGE 2 ) were studied in human skin. In addition, the effects of capsaicin were examined as it is known to release (and to deplete) SP and calcitonin gene‐related peptide from C‐fibres. The flare evoked by bradykinin was abolished by pretreatment with lignocain (local anaesthetic), compound 48/80 (mast‐cell histamine liberator), mepyramine (H 1 ‐receptor antagonist) and indomethacin (cyclo‐oxygenase inhibitor) but was unaffected by atropine and ketanserin (serotonin antagonist). The weal response was not reduced by any of the drugs. The flare evoked by capsaicin was abolished by lignocaine and indomethacin but was unaffected by compound 48/80, mepyramine, atropine and ketanserin. The weal response was reduced by indomethacin. The flare response to bradykinin seems to reflect the activation of C‐fibres and associated mast cells, while the flare response to capsaicin seems to reflect the activation of C‐fibres only. Repeated injections of capsaicin and bradykinin produced tachyphylaxis (and cross‐tachyphylaxis) and greatly reduced the SP‐evoked flare. Capsaicin produced tachyphylaxis also after treatment of the skin with a local anaesthetic, suggesting that it develops independently of C‐fibre impulse flow. The tachyphylaxis produced by bradykinin and capsaicin seems to reflect the depletion of messenger peptides from the C‐fibres. The flare response to SP following capsaicin‐ or bradykinin‐induced desensitization gradually returned to normal after 5–8 weeks. The erythema evoked by PGE 2 was reduced by 30% following pretreatment with lignocaine, mepyramine or compound 48/80. The dermal effects of prostaglandins seem to be partly indirect, involving C‐fibres and mast cells, and partly direct on the vascular bed. The importance of prostaglandins for C‐fibre‐mediated effects is reflected in the fact that indomethacin and acetylsalicylic acid were found to suppress axon‐reflex‐mediated responses.