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Inhibitory effect of human fibroblast interferon (HuIFN‐ β ) on the growth and invasive potential of cultured human melanoma cells in vitro
Author(s) -
FUKUZAWA K.,
HORIKOSHI T.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb00673.x
Subject(s) - in vitro , melanoma , fibroblast , interferon , cancer research , inhibitory postsynaptic potential , cell culture , biology , medicine , immunology , virology , biochemistry , genetics
Summary The effect of HuIFN‐ β on the invasive potential of melanoma cells was studied using an in‐vitro model system with Transwell chambers equipped with matrigel‐coated polycarbonate filters. When (10 2 , 10 3 and 10 4 IU/ml) for 3 days and then grown in medium without HuIFN‐ β for another 7 days. On day 7, the proliferation of melanoma cells was inhibited by 77 and 87%, respectively, when cells were treated with 10 2 and 10 4 IU/ml of HuIFN‐ β . This antiproliferative effect was dose‐dependent and more pronounced on day 11. The effect of HuIFN‐ β on the invasive potential of melanoma cells was studied using an in‐vitro model system with Transwell chambers equipped with Matrigel‐coated polycarbonate filters. When cells were treated with HuIFN‐ β (10 2 , 10 3 and 10 4 IU/ml) for 24 h, the amount of tritiated thymidine incorporated into cells was increased, indicating that cell growth was not inhibited. However, the number of cells that invaded to the filter decreased significantly by 15–40%. HuIFN‐ β did not have an inhibitory effect on the haptotactic migration of melanoma cells. These data indicate that the antiproliferative effect of HuIFN‐ β occurs after 24 h and that the direct anti‐invasive effect is independent of any effect on proliferation.