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1α,25‐dihydroxyvitamin D 3 modulates Ia antigen expression induced by interferon‐γ and prostaglandin E 2 production in Pam 212 cells
Author(s) -
TANI M.,
KOMURA A.,
HORIKAWA T.
Publication year - 1992
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1992.tb00657.x
Subject(s) - prostaglandin e2 , interferon γ , antigen , interferon , chemistry , immunology , endocrinology , medicine , microbiology and biotechnology , interferon gamma , biology , cytokine
Summary Recent investigations suggest that 1α,25‐dihydroxyvitamin D 3 [1α,25(OH) 2 D 3 ] has an effect on the regulation of immune responses and that keratinocyte (KC) expression of major histocompatibility complex class II antigens may be involved in immune responses. We investigated the modulation by 1α,25(OH) 2 D 3 of Ia antigen expression induced by interferon‐γ (IFN‐γ) and prostaglandin E 2 (PGE 2 ) production in Pam 212 cells. 1α,25(OH) 2 D 3 at 10 −6 , 10 −8 or 10 −10 m significantly decreased the levels of IFN‐γ‐induced Ia antigen expression in Pam 212 cells. Pam 212 cells produced PGE 2 and 1α,25‐(OH) 2 D 3 enhanced Pam 212 cell PGE 2 production. However, indomethacin (1, 5 and 10 μg/ml) did not abrogate the inhibitory effect of 1α,25(OH) 2 D 3 on IFN‐γ induction of Ia antigen expression in Pam 212 cells, indicating that the products of the cyclo‐oxygenase pathway do not mediate 1α,25(OH) 2 D 3 inhibition of IFN‐γ induction of Pam 212 cell Ia antigen expression. Our studies suggest that in Pam 212 cells the levels of Ia antigen expression induced by IFN‐γ and PGE 2 production are negatively and positively regulated by 1α,25(OH) 2 D 3 , respectively, and that 1α,25(OH) 2 D 3 may play a role in the regulation of immune responses.