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Detection of a novel basement membrane antigen by GDA‐J/F3 anti‐human sperm fibrous sheath monoclonal antibody
Author(s) -
JASSIM A.,
SCHOFIELD O.,
WHITEHEAD P.,
PURKIS P.,
HEAGERTY A.H.M.,
SACHS J.A.,
EADY R.A.J.,
LEIGH I.M.
Publication year - 1991
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1991.tb06055.x
Subject(s) - biology , immunoperoxidase , pathology , immunoelectron microscopy , antigen , basement membrane , lamina densa , submucosal glands , monoclonal antibody , antibody , epithelium , anatomy , immunology , medicine
Summary Basement membrane zones (BMZ) of human epithelia were stained with GDA‐J/F3 monoclonal antibody, which was originally raised against sperm cells. Using indirect immunofluorescence and immunoperoxidase techniques, the antibody reacted with the BMZ of stratified squamous epithelia (skin and its appendages, tongue, lip, oesophagus and cervix). It also stained the BMZ of trachea, nasal ciliated mucosa, some mammary ducts of lactating and resting breast, amnion and ureter but failed to react with that of stomach, ileum. colon, rectum, kidney, liver, fallopian tube, lung or their blood vessels. In testes, the antibody did not react with the BMZ of the seminiferous tubules although the sperm tails were stained. Split‐skin immunofluorescence and immunoelectron microscopy localized GDA‐J/F3 antigen to the inferior border of the lamina densa of the BMZ. In human foetuses, the epidermally associated antigen was detected at an estimated gestational age of 9 weeks, and in the amnion at 1 5 weeks. The antibody reacted with tissues from monkey but not from mouse, rat, cow or pig suggesting the late appearance of the antigen during evolution. Although the GDA‐J/F3 was difficult to characterize biochemically, its tissue distribution, ontogeny and ultrastructural localization suggests that this antigen may be a type VII collagen‐associated protein, whose expression is altered in recessive dystrophic epidermolysis bullosa. This disease could represent abnormalities in type VII collagen structure, assembly, transport or interaction with associated proteins.