Premium
In‐vitro DNA synthesis of keratinocytes in normal human skin, psoriasis, seborrhoeic keratosis, Bowen's disease and basal cell carcinoma
Author(s) -
MORIMOTO Y.,
SAGA K.,
BANDO M.,
TAKAHASHI M.
Publication year - 1991
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1991.tb06030.x
Subject(s) - bowen's disease , psoriasis , seborrhoeic dermatitis , basal cell carcinoma , dermatology , keratosis , actinic keratosis , basal cell , human skin , in vitro , seborrheic keratosis , medicine , dna , pathology , darier's disease , keratinocyte , cancer research , disease , biology , genetics
Summary The DNA‐synthesizing cells in benign and malignant keratotic diseases including psoriasis, seborrhoeic keratosis, Bowen's disease and basal cell carcinoma were studied. Cells in S‐phase were labelled after small pieces of the lesions were incubated in tissue culture medium that contained 5‐bromo‐2′‐deoxyuridine (BrdU). The labelling of DNA was analysed using the labelling index and pattern. Although the malignant diseases tended to show a higher DNA labelling index, neither this nor the loss of polarity alone was associated with malignancy. However, a higher DNA labelling index together with a loss of polarity was characteristic for malignancy.