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Histamine release from rodent and human mast cells induced by protoporphyrin and ultraviolet light: studies of the mechanism of mast‐cell activation in erythropoietic protoporphyria
Author(s) -
GLOVER RENEE A.,
BAILEY CYNTHIA S.,
BARRETT KIM E.,
WASSERMAN S.I.,
GIGLI IRMA
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb14727.x
Subject(s) - histamine , erythropoietic protoporphyria , mast cell , prostaglandin d2 , ultraviolet light , catalase , protoporphyrin , calcium , chemistry , peritoneal cavity , microbiology and biotechnology , biology , immunology , biochemistry , endocrinology , prostaglandin , oxidative stress , porphyrin , photochemistry , organic chemistry , anatomy
SUMMARY We report that protoporphyrin (PP) and ultraviolet light (UVA) induces histamine release from rat peritoneal mast cells, mouse bone marrow mast cells and human cutaneous mast cells in a dose‐ and temperature‐dependent manner. The mast‐cell activation was associated with loss of membrane integrity and inhibited by the hydrogen peroxide scavenger, catalase. Histamine release was independent of extracellular calcium in the rodent mast cells, but was markedly reduced in the absence of calcium in human cells. These findings indicate that PP and UVA induce mast‐cell‐mediator release by a process that may involve hydrogen peroxide formation. There appear to be differences in response to PP and UVA between rodent and human mast cells.