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The discrimination between nickel‐sensitive and non‐nickel‐sensitive subjects by an in vitro lymphocyte transformation test
Author(s) -
EVERNESS K.M.,
GAWKRODGER D.J.,
BOTHAM P.A.,
HUNTER J.A.A.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb08276.x
Subject(s) - nickel , stimulation , in vitro , nickel compounds , medicine , lymphocyte , reactivity (psychology) , chemistry , endocrinology , immunology , biochemistry , pathology , organic chemistry , alternative medicine
SUMMARY A lymphocyte transformation test (LTT) was able to distinguish between nickel‐sensitive subjects and non‐nickel‐sensitive controls. Sixty‐one out of 66 (92%) nickel‐sensitive subjects had positive stimulation indices in 6‐ and/or 7‐day assays using 5 μg/ml of nickel sulphate, whereas none of the 43 controls gave positive results. Stimulation indices were not enhanced by the patch testing of subjects to nickel before performing the LTT. A weak correlation was seen between the results of the LTT and the macroscopic degree of patch‐test reactivity. The concentration of nickel sulphate used (5 μg/ml) did not have a significant non‐specific mitogenic effect.