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Human normal‐resting epidermal Langerhans cells do express the type 3 complement receptor
Author(s) -
PANFILIS G.,
SOLIGO D.,
MANARA G.C.,
FERRARI C.,
TORRESANI C.,
ZUCCHI A.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb08258.x
Subject(s) - immunogold labelling , immunostaining , langerhans cell , electron microscope , epidermis (zoology) , human skin , biology , chemistry , microbiology and biotechnology , immunocytochemistry , pathology , biophysics , ultrastructure , immunohistochemistry , endocrinology , immunology , anatomy , antigen , medicine , optics , physics , genetics
SUMMARY The expression of CR3 by murine‐resting epidermal Langerhans cells (LC) is well established, but CR3 expression by human normal‐resting epidermal LC has not yet been demonstrated. In this study, highly sensitive immunostaining techniques, such as immunogold labelling in transmission‐ and scanning‐electron microscopy, were used on freshly isolated, LC‐enriched, normal human epidermal cells. Human normal resting epidermal LC were found to be CR3 + , since a low but significant number of gold granules labelled the plasma membrane of all the LC observed under transmission‐electron microscopy, and all the epidermal cells showing LC morphology as observed by scanning‐electron microscopy.