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Immunomodulating cytokines in atopic dermatitis and psoriasis: production of tumour necrosis factor and lymphotoxin by mononuclear cells in vitro
Author(s) -
KAPP A.,
TEXTOR A.,
KRUTMANN J.,
MöLLER A.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb07279.x
Subject(s) - lymphotoxin , peripheral blood mononuclear cell , atopic dermatitis , psoriasis , tumor necrosis factor alpha , medicine , immunology , lymphotoxin beta receptor , lymphotoxin alpha , tumor necrosis factor α , in vitro , necrosis , dermatology , biology , pathology , biochemistry
SUMMARY The immunomodulating cytokines, tumour necrosis factor/cachectin (TNF) and lymphotoxin (LT) are thought to play an essential role as mediators of inflammatory reactions. To evaluate the role of TNF and LT in atopic dermatitis (AD) and psoriasis, we investigated their production by mononuclear cells (MNC) in vitro . The 24‐h supernatants of lipopolysaccharide (LPS)‐ and phytohaemagglutinin (PHA)‐stimulated and unstimulated MNC from 26 patients with AD and 20 with psoriasis and from 17 non‐atopic healthy controls were tested for the concentrations of TNF and LT using an ELISA technique. In patients with AD, TNF levels were significantly decreased in the supernatant of PHA‐stimulated (P 0·005) and LPS‐stimulated (P0·02) MNC in comparison to controls. There was no significant difference in TNF production between psoriatic patients and the control group. Release of LT in the supernatant of PHA‐stimulated MNC by patients and controls did not differ significantly. There was no significant spontaneous production of TNF and LT by MNC of patients and controls. These studies indicate that different immunomodulating mechanisms are responsible for triggering the inflammatory response in AD and psoriasis.