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Invasive migration of epidemic Kaposi's sarcoma cells in vitro
Author(s) -
SCHIRREN C.G.,
ROTH W.K.,
HEIN R.,
WERNER S.,
KRIEG T.,
BRAUNFALCO O.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb06290.x
Subject(s) - matrigel , sarcoma , in vitro , basement membrane , chemotaxis , connective tissue , pathology , immunology , kaposi's sarcoma , medicine , biology , biochemistry , receptor , human herpesvirus
SUMMARY Kaposi's sarcoma (KS) is a low grade malignant neoplasm which shows invasive growth and often occurs in immunosuppressed patients with the Acquired Immune Deficiency Syndrome (AIDS; epidemic KS). It is also found in elderly men where it is usually limited to the skin (classic KS). The present study investigated the chemotaxis and invasive migration of epidemic KS cells in vitro and compared them to cells grown from classic KS lesions and to fibroblasts. Epidemic KS cells demonstrated invasive migration through reconstituted basement membrane (Matrigel) as well as through interstitial connective tissue (collagen I) in early passages, whereas fibroblasts did not invade either barrier. Epidemic KS cells in late passages did not show any invasive migration. Following pretreatment with tumour necrosis factor α (TNF‐α) there was no enhanced migration through the Matrigel and collagen I for epidemic KS cells, whereas classic KS cells showed an increased migration through the type I collagen barrier.