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Production of antikeratin autoantibodies by hybrid spleen cells of naive mice
Author(s) -
IWATSUKI K.,
IMAIZUMI S.,
HASHIZUME H.,
SUGAYA K.,
TAKIGAWA M.,
YAMADA M.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb04190.x
Subject(s) - biology , antibody , spleen , keratin , microbiology and biotechnology , immunofluorescence , western blot , antigen , cell culture , virology , immunology , paleontology , biochemistry , genetics , gene
SUMMARY The mechanism of the occurrence of natural antikeratin antibodies in human sera was studied using hybrid spleen cells obtained from experimentally naive or from immunized mice. Antikeratin antibodies were detected by enzyme‐linked immunosorbent assay (ELISA) in 5·9–9·5% of the culture supcrnatants of fused spleen cells taken from naive mice. When mice were immunized with keratins, the number of supernatants containing antikeratin antibodies was increased to eight out of 51 (15.7%). When immunized with non‐keratin materials such as activated human T cells, adult T‐cell leukaemia cell lysates, and human T‐cell lymphotropic virus type‐1 (HTLV‐1), 16·7–20·8% of the supernatants were found to contain antikeratin antibodies by ELISA. The antikeratin antibodies in the supernatants showed cytoplasmic staining of keratinocytes in human as well as mouse skin by indirect immunofluorescence. The antibodies reacted with extracted human epidermal keratins by dot‐blot and Western blot analysis. Most antikeratin antibodies in the supernatants did not show cross‐reactivity with exogenous antigens used for immunization and vimentin‐type intermediate‐sized filaments. The findings demonsrate that B cells producing antikeratin antibodies are common in naive mice, and produce various type of antikeratin antibodies following specific activation with epidermal keratins and non‐specific immunological stimuli.