Premium
Integrins of normal human epidermis: differential expression, synthesis and molecular structure
Author(s) -
KLEIN C.EBERHARD,
STEINMAYER T.,
MATTES J.M.,
KAUFMANN R.,
WEBER L.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb01844.x
Subject(s) - epidermis (zoology) , integrin , microbiology and biotechnology , differential (mechanical device) , chemistry , biology , pathology , computational biology , biochemistry , medicine , anatomy , cell , physics , thermodynamics
summary The expression of integrin cell surface receptors in normal skin and their synthesis and molecular structure in keratinocyte cultures was investigated. The reactivity of four different polypeptides of the integrin family (α 2 ‐,α 3 ‐,α 6 ‐ and β 1 ‐chains) was demonstrated in the basal cell layer of normal epidermis. Studies of labelled keratinocyte cell lines showed that the polypeptides were expressed as α 2 β 1 , α 3 β 1 and α 6 β 4 integrins. Only the α 6 β 4 integrin showed polarization towards the basement membrane attachment site of basal layer keratinocytes, and was preferentially expressed at microvillous projections. In contrast, α 2 β 1 and α 3 β 1 integrins were equally expressed throughout the basal cell plasma membrane.