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Growth‐inhibitory effects of 1,25‐dihydroxyvitamin D 3 on normal and psoriatic keratinocytes
Author(s) -
HASHIMOTO K.,
MATSUMOTO K.,
HIGASHIYAMA M.,
NISHIDA Y.,
YOSHIKAWA K.
Publication year - 1990
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1990.tb01828.x
Subject(s) - psoriasis , keratinocyte , epidermis (zoology) , calcitriol , microbiology and biotechnology , dna synthesis , endocrinology , chemistry , dermatology , cancer research , medicine , biology , dna , in vitro , biochemistry , anatomy , vitamin d and neurology
summary The effects of 1,25‐dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ) on the growth and DNA synthesis of cultured human keratinocytes obtained from involved and uninvolved psoriatic epidermis and normal epidermis were studied. Treatment with 10 ‐8 m and 10 ‐7 m of 1,25(OH) 2 D 3 inhibited cell growth as follows: 58.5±19.3% and 21.3±13.6% in normal keratinocytes ( n =6); 43.8±22.8% and 17.8±12.3% in psoriatic uninvolved keratinocytes ( n =4); 51.7±18.2% and 13.2±6.4% in psoriatic involved keratinocytes ( n =6). Inhibition was virtually complete at 10 ‐6 m . DNA synthesis was also inhibited by 10 ‐8 m , 10 ‐7 m and 10 ‐6 m of 1,25(OH) 2 D 3 as follows: 70.0±8.3%, 59.0±6.8% and 16.7±4.0%, respectively, in normal keratinocytes ( n =3); 78.5±13.5%, 51.5±25.5% and 24.5±21.5%, respectively, in psoriatic uninvolved keratinocytes ( n =2); and 69.3±14.5%, 41.3±19.1% and 14.8±11.2%, respectively, in psoriatic involved keratinocytes ( n =4). These results indicate that 1,25(OH) 2 D 3 functions as a growth inhibitor for cultured human keratinocytes derived from both normal and psoriatic skin.