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Effect of human recombinant interleukin‐Iα on release of prostacyclin from human endothelial cells
Author(s) -
RUSTIN M.H.A.,
BULL HELEN A.,
DOWD PAULINE M.
Publication year - 1989
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1989.tb07779.x
Subject(s) - prostacyclin , cycloheximide , incubation , endocrinology , tachyphylaxis , medicine , prostaglandin antagonist , prostaglandin , chemistry , pharmacology , biology , immunology , biochemistry , protein biosynthesis
SUMMARY Incubation of human recombinant IL‐1α (hrIL‐1α) with cultured human endothelial cells induced a dose‐and time‐dependent increase in the release of prostacyclin (PGI 2 ). Above a dose of hrIL‐1α 0.05 units/ml and following a variable lag phase of between 2 and 4 h, PGI 2 release (measured as the stable hydrolysis product 6‐keto‐prostaglandin F 1α ) was detected in the culture supernatant and levels continued to rise throughout a 48‐h incubation. The release of PGI 2 required the continued presence of hrIL‐1α, did not demonstrate tachyphylaxis and was not reduced by pre‐incubation with the protein synthesis inhibitors cycloheximide, tunicamycin and actinomycin or by the calmodulin antagonist trifluoroperazine. The relationship of these results to ultraviolet radiation induced erythema is discussed.