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1α‐25‐dihydroxyvitamin D 3 increases intracellular free calcium in murine B16 melanoma
Author(s) -
HILL S.E.,
BLEEHEN S.S.,
MACNEIL S.
Publication year - 1989
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1989.tb07761.x
Subject(s) - intracellular , extracellular , calcium , vitamin d and neurology , calcium in biology , endocrinology , vitamin , metabolite , alpha (finance) , medicine , chemistry , cholecalciferol , calcitriol , active metabolite , biology , biochemistry , surgery , construct validity , patient satisfaction
SUMMARY Vitamin D 3 and its active metabolite 1α‐25‐dihydroxyvitamin D 3 (1α‐25‐(OH) 2 D 3 ) have been reported to play a role in melanogenesis. Physiological concentrations of 1α‐25‐(OH) 2 D 3 ) were found to acutely elevate intracellular free calcium (using Fura 2) in B16 primary (1°) cells. Membrane phosphoinositide turnover was unaffected by 1α‐25‐(OH) 2 D 3 . The rise in intracellular free calcium was entirely dependent on extracellular calcium and was not mimicked by vitamin D 3 . However, in neither B16‐1° nor B16‐F 1 melanoma cells did vitamin D 3 or 1α‐25‐(OH) 2 D 3 increase melanin production.