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DNA analysis by flow cytometry in cutaneous T‐cell lymphomas
Author(s) -
RALFKIAER ELISABETH,
LARSEN JØRGEN K.,
CHRISTENSEN IB J.,
THOMSEN KRISTIAN,
WANTZIN GUNHILD LANGE
Publication year - 1989
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1989.tb01343.x
Subject(s) - flow cytometry , dna , pathology , medicine , dermatology , biology , immunology , genetics
SUMMARY DNA histograms of skin and blood specimens from 64 patients with known or suspected cutaneous T‐cell lymphoma (CTCL) have been examined and compared with normal blood mononuclear cells and skin biopsy samples from 50 patients with various benign cutaneous conditions (i.e. patch test infiltrates, eczema, psoriasis, lichen planus, atopic dermatitis) in an attempt to establish whether DNA measurements by flow cytorntry may improve the early recognition of CTCL. The results indicate that right‐skewed G0/G1 peaks are seen frequently in both benign disorders and known and suspected CTCL. Such peaks may reflect increased stainability of DNA due to chromatin dispersion during cell activation and/or cell proliferation and do not constitute reliable evidence of malignancy. In contrast, discrete aneuploid DNA peaks are confined to malignant lesions, but are seen almost exclusively in the advanced stages in which the diagnosis can be establignohed easily based on routine histological criteria. These data indicate that DNA measurements by flow cytometry is of only limited help in the early recognition of CTCL and support the view that the lymphoid infiltrate in early CTCL may be reactive (rather than neoplastic) or alternatively may contain only minor populations of abnormal (malignant) cells which cannot be detected by currently available DNA measurement techniques.