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Inhibition of proliferation of HeLa cells by dithranol (anthralin) and 10‐butyryl dithranol (butantrone)
Author(s) -
REMITZ ANITA,
KARINIEMI ARJALEENA,
LEHTONEN EERO,
NORDLING STIG
Publication year - 1989
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1989.tb01326.x
Subject(s) - dithranol , dermatology , medicine , chemistry , psoriasis
SUMMARY The effects were studied of two antipsoriatic drugs, dithranol (anthralin) and butantrone on the proliferation of HeLa cells. Non‐confluent monolayers of HeLa cells were treated with various concentrations (10 −5 ‐10 −8 M) of dithranol or butantrone for 45 min. The cells were then cultivated for up to 48 h and treated with 10 μM bromodeoxyuridine (BrdU) and 10 μM fluorodeoxyuridine for 30 min before harvesting. Both drugs at concentrations of 10 −6 and 10 −5 M inhibited the BrdU uptake of HeLa cells, but did not cause any significant change in the proportion of cells in S‐phase. The inhibitory effect of dithranol on cell number was linear with concentration 24 h after incubation. The highest (10 −5 M) concentration of butantrone caused an abrupt decrease in cell number.