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Calcium/calmodulin regulation of the proliferation of human epidermal keratinocytes, dermal fibroblasts and mouse B16 melanoma cells in culture
Author(s) -
ALANI A. M.,
MESSENGER A. G.,
LAWRY J.,
BLEEHEN S. S.,
MACNEIL SHEILA
Publication year - 1988
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1988.tb03221.x
Subject(s) - calmodulin , keratinocyte , calcium , epidermis (zoology) , fibroblast , microbiology and biotechnology , calcium in biology , cell growth , cell culture , biology , extracellular , intracellular , chemistry , medicine , biochemistry , anatomy , genetics
SUMMARY We have investigated the relationship between extracellular calcium, intracellular calmodulin and proliferation in normal keratinocytes. Keratinocyte proliferation and its sensitivity to calmodulin antagonists was compared with that of normal human dermal fibroblasts and neoplastic mouse B16 melanoma cells. Keratinocytes were similar to fibroblasts in showing reduced proliferation in low (0‐15 mM) calcium medium and unlike B16 cells which continued to proliferate until calcium was reduced to submicromolar levels. Intracellular calmodulin was significantly higher in rapidly dividing keratinocytes in normal (1–15 mM) calcium medium than in slower dividing cells in low (0–15 mM) calcium. Fibroblasts and B16 cells maintained similar calmodulin levels in both low and normal calcium media. Calmodulin antagonists inhibited proliferation of all three cell types equally. Thus, keratinocyte calmodulin seems related to the proliferative state of the cell (unlike fibroblast calmodulin) and calmodulin antagonists may be of use in controlling the hyperpro‐liferation of the psoriatic epidermis.