Premium
Characterization of specific leukotriene C 4 binding sites on cultured human keratinocytes
Author(s) -
MULLER A.,
MICHEL L.,
BASSETSEGUIN N.,
MODAT G.,
DUBERTRET L.,
BONNE C.
Publication year - 1988
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1988.tb03218.x
Subject(s) - leukotriene b4 , leukotriene , leukotriene c4 , receptor , binding site , chemistry , human skin , biology , immunology , inflammation , biochemistry , genetics , asthma
SUMMARY Peptidoleukotriene C 4 (LTC 4 ) and leukotriene B 4 (LTB 4 ) are both suspected of being inflammatory mediators and epidermal mitogenic agents in cutaneous psoriatic lesions. In the present study an LTC 4 specific binding site was characterized in membranes from cultured human keratinocytes (K d , 8.7 nmol/1; B max , 1.2 pmol/mg protein). In contrast LTB 4 did not show any high affinity binding which could account for its biological effects. These data suggest that LTC 4 , unlike LTB 4 , acts on epidermal cells through a receptor‐mediated mechanism.