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Localization of cutaneous antigens by the immunogold‐silver staining technique
Author(s) -
ALLEN M.H.,
TIDMAN M.J.,
MACDONALD D.M.
Publication year - 1988
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1988.tb02565.x
Subject(s) - immunogold labelling , staining , antigen , pathology , biology , medicine , microbiology and biotechnology , chemistry , immunology , ultrastructure
SUMMARY Immunogold‐silver staining (IGSS) was evaluated for the localization of cutaneous antigens in frozen and paraffin‐embedded tissue, employing antibodies to a variety of intracellular, cell‐surface and extracellular epitopes in an indirect immunogold reaction, followed by silver enhancement. The principal advantages of IGSS are the avoidance of toxic reagents and theproduction of a silver precipitate that is permanent, clearly visible and of sufficient contrast with the reaction products of alternative immunolocalizing techniques to be of value in double‐labelling procedures. It seems to localize antigenic determinants well, but does not appear to be particularly sensitive, especially for the demonstration of antibodies to extracellular constituents. Limitations of IGSS include the propensity to non‐specific staining, and the need to vary the duration of the devcJopment stage during silver enhancement.