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Effect of a platelet release fraction on glycosaminoglycan synthesis by cultured dermal fibroblasts from patients with progressive systemic sclerosis
Author(s) -
FALANGA V.,
ALSTADT STEPHANIE P.
Publication year - 1988
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1988.tb02426.x
Subject(s) - glycosaminoglycan , chemistry , progressive systemic sclerosis , platelet , medicine , cancer research , immunology , biochemistry , raynaud disease
SUMMARY Confluent cultures of dermal fibroblasts from patients with progressive systemic sclerosis (PSS) and healthy controls were investigated for the synthesis of glycosaminoglycans (GAG) in response to a platelet release fraction (PRF) obtained by treatment of pooled platelets from normal individuals with adenosine 5′‐diphosphate (ADP). PRF, at concentrations of 1 to 50 μg protein/ml, produced a linear increase in GAG synthesis that was always greater in cultures of PSS fibroblasts than in cultures of normal fibroblasts (P < 0·001). A partial inhibition of GAG synthesis was observed with 100 μg/ml of PRF. The increased GAG synthesis in cultures incubated with PRF was not due to ADP. These findings demonstrate a difference in response to PRF between PSS and normal fibroblasts and may reflect an increased responsiveness of PSS fibroblasts to platelet factors.