Premium
Flow cytometric quantification of T6‐positive cells in psoriatic epidermis after PUVA and methotrexate therapy
Author(s) -
GOMMANS J. M.,
HEZIK S. J. T. M.,
HUYSTEE B. E. W. L.
Publication year - 1987
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1987.tb05899.x
Subject(s) - epidermis (zoology) , methotrexate , puva therapy , trypsinization , psoriasis , medicine , dermatology , flow cytometry , pathology , chemistry , immunology , enzyme , anatomy , biochemistry , trypsin
SUMMARY We describe a flow cytometric technique for measuring the percentage of T6‐positive cells in suspensions prepared by trypsinization of human epidermis. The value obtained for healthy controls (1.55 ± 0.52%) corresponds to about 600 T6‐positive cells per mm2 of skin surface, a figure in line with histological estimates. No significant change was found in the percentage of T6‐positive cells in either the uninvolved or lesionat epidermis of untreated psoriatic patients. PUVA treatment resulted in a significant reduction in T6‐positive cells. A cyclic fluctuation in the numbers of T6‐positive cells was shown to accompany methotrexate administration on a weekly divided dose schedule.