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Cytochemical localization of VIP‐stimulated adenylate cyclase activity in human sweat glands
Author(s) -
TAINIO HANNA
Publication year - 1987
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1987.tb05845.x
Subject(s) - cyclase , adenylate kinase , vasoactive intestinal peptide , endocrinology , medicine , receptor , chemistry , acetylcholine , biology , neuropeptide
SUMMARY Vasoactive intestinal polypeptide (VlP)‐stimulated adenylate cyclase was demonstrated cytochcmically in human sweat glands. Biopsies containing axillary sweat glands were incubated with 5 μm VIP and adenylate cyclase was demonstrated using the cerium‐salt technique. Reaction products were localized in the plasma memhranes of glandular and myoepithelial cells. Thus, VIP appears to stimulate adenylate cyclase activity without acetylcholine. VIP and acetylcholine in human sweat glands most probably act through a receptor complex, where adenylate cyctase is coupled to that part of the receptor stimulated by VIP.