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The effect of altering area of application and dose per unit area on sensitization by DNCB
Author(s) -
WHITE S. I.,
FRIEDMANN P. S.,
MOSS CELIA,
SIMPSON JUDY M.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb06646.x
Subject(s) - newcastle upon tyne , newcastle disease , white (mutation) , medicine , dermatology , history , art history , biology , biochemistry , virus , virology , gene
SUMMARY We have investigated the contributions of dose per unit area and area of application to the degree of sensitivity induced by dinitrochlorobenzene (DNCB). Subjects sensitized with 35.4 μg/cm 2 showed equal sensitization whether the total dose was 250 μg applied to 7.1 cm 2 or 62.5μg applied to 1.8 cm 2 . Three groups of subjects were sensitized with 16.4 μg/cm 2 applied as 56μg on 3.5 cm 2 , 116μg on 7.1 cm 2 or 232μg on 14.2cm 2 . Although the total dose changed by a factor of four, the proportions of subjects sensitized were comparable (66, 50 and 55% respectively), and their degrees of reactivity were similar. This contrasts with previous findings that when the area of sensitization was fixed and the concentration was increased over a similar range, highly significant increases in the proportion of sensitized subjects and degree of reactivity occurred.