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Incontinentia pigmenti: eosinophil chemotactic activity of the crusted scales in the vesiculobullous stage
Author(s) -
TAKEMATSU H.,
TERUI T.,
TORINUKI W.,
TAGAMI H.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb06220.x
Subject(s) - eosinophil , chemotaxis , leukotriene b4 , sephadex , eosinophilia , incontinentia pigmenti , infiltration (hvac) , immunology , chemistry , microbiology and biotechnology , pathology , enzyme , biochemistry , biology , medicine , inflammation , physics , receptor , asthma , thermodynamics
SUMMARY To investigate the mechanisms underlying eosinophil infiltration into the epidermis in incontinentia pigmenti (IP), we studied the eosinophil chemotactic activity in extracts of the crusted scales from three patients with IP in the vesiculobullous stage. Eosinophil ehemotactic activity was detected in the eluates from a Sephadex G‐75 chromatography column between the vitamin B 12 and phenol red markers. The chemotactic activity was heat‐stable and resistant to enzyme digestion, and recovered after ether extraction at low pH. Leukotriene B4 (LTB4) was demonstrated in the fractions with high eosinophil chemotactic activity. These findings suggest that LTB4 plays an important role in the accumulation of eosinophils within the epidermis in IP, in the vesiculobullous stage. Blood eosinophilia, however, may not be induced by the eosinophil chemotactic factors in the scales.