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Monoclonal antibodies against epidermal cell surface and dermal‐epidermal junction: antigens which may be epidermal cell spreading factors
Author(s) -
KATAYAMA H.,
KINO J.,
ITAMI S.,
TSUTSUI M.,
KOIZUMI H.,
HALPRIN K.M.,
ADACHI K.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb06215.x
Subject(s) - antigen , monoclonal antibody , epidermis (zoology) , microbiology and biotechnology , keratin , keratinocyte , antibody , chemistry , biology , cell , cell culture , immunology , biochemistry , anatomy , genetics
SUMMARY Two monoclonal antibodies which reacted with the epidermal cell surface (SF‐1) and the dermal‐epidermal‐junction (SF‐2), respectively, were obtained by immunizing mice with partially‐purified human epibolin. The corresponding antigens were partially purified from fetal bovine serum by affinity chromatography using these antibodies. SDS‐polyacrylamide gel electrophoresis showed that these antigens contained polypeptide components with molecular weights different from that of epibolin (mol. wt. 65000 daltons); SF‐1 antigen had a 68000 dalton main component, and SF‐2 antigen a broad 58000–61000 dalton main component. Both of these partially‐purified antigens promoted the spreading of dissociated pig epidermal cells. SF‐2 antigen also promoted the spreading of Pam cells (a murine keratinocyte line). The results suggest that proteins capable of promoting epidermal cell spreading may be present on the epidermal cell surface and at the dermal‐epidermal junction. However, their physiological role in keratinization remains to be elucidated.