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Support and stimulation of epidermal cell outgrowth from porcine skin explants by platelet factors
Author(s) -
HEBDA PATRICIA A.,
ALSTADT STEPHANIE P.,
HILEMAN W.T.,
EAGLSTEIN W.H.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb05762.x
Subject(s) - stimulation , explant culture , microbiology and biotechnology , platelet , cell , medicine , immunology , chemistry , biology , in vitro , biochemistry
SUMMARY The effect of a platelet homogenate fraction (PHF) on epidermal cell outgrowth from porcine skin explants was examined in culture medium with and without 5% foetal bovine serum (FBS). Explants were sustained and outgrowth was initiated, supported and stimulated by PHF, in the absence of FBS. PHF‐supported outgrowth was concentration‐dependent up to 130 μg protein/ml. PHF activity was not removed by heat treatment at 100° C for 2 min but was destroyed by heating at 100° C for 5 min. The factor responsible for epidermal cell outgrowth in PHF may be epibolin or serum spreading factor, or may be a platelet‐derived factor as yet undescribed. Viability and outgrowth were stimulated by the ‘high molecular weight’ fraction of PHF (>30 kd). PHF plus 5% FBS gave inhibition of outgrowth, which was not PHF concentration‐dependent; however, this inhibitory effect was variable with different PHF preparations. This study is the first to demonstrate an effect of platelet‐derived factors on epidermal cell viability and outgrowth.