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Ionophore A23187 stimulates arachidonic acid release and cyclic GMP accumulation in pig epidermis
Author(s) -
KATO N.,
SUYA H.,
AOYAGI T.,
TAYLOR J.R.,
HALPRIN K.M.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb05744.x
Subject(s) - arachidonic acid , ionophore , cyclic gmp , epidermis (zoology) , chemistry , endocrinology , medicine , microbiology and biotechnology , biochemistry , biology , anatomy , membrane , enzyme
SUMMARY We studied the effect of Ionophore A23187 (A23187) on phospholipid degradation and the accumulation of cyclic GMP in pig epidermis. A23187 stimulated the release of AA, probably partially through the activation of phospholipase A. A23187 also stimulated the accumulation of epidermal cyclic GMP, but the activity of cyclic GMP‐dependent phosphodiesterases was not altered. Mepacrine, an inhibitor of phospholipase A * , inhibited the A23i87‐stimulated accumulation of cyclic GMP. The results suggest that A23i87 stimulates the accumulation of epidermal cyclic GMP by a calcium‐dependent process which also requires products of phospholipid degradation.