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Secretion of plasminogen activator inhibitor by cultured human epidermal cells
Author(s) -
HASHIMOTO K.,
NISHIOKA K.,
KATAYAMA I.,
YOSHIKAWA K.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb05708.x
Subject(s) - secretion , plasminogen activator , activator (genetics) , chemistry , medicine , endocrinology , cancer research , microbiology and biotechnology , biology , receptor
SUMMARY A conditioned medium from cultured human epidermal cells was observed to inhibit the activity of exogenous urokinase. By reverse fibrin autography after SDS polyacrylamide gel electrophoresis, a plasminogen activator inhibitor was detected with a molecular weight of 46000. Using Mr‐ 33000 [ 125 I]‐labelled urokinase we observed the formation of an enzyme‐ligand complex. The molecular weight of this complex was 79000. These results indicate that cultured human epidermal cells secrete a plasminogen activator inhibitor (urokinase inhibitor) with a molecular weight of 46000.