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HB8: a monoclonal antibody recognizing elastic fibre microfibrils
Author(s) -
SCHMITT D.,
CHIGNOL MARIECHRISTINE,
BROCHIER J.,
THIVOLET J.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb04873.x
Subject(s) - immunogold labelling , ultrastructure , electron microscope , elastin , reactivity (psychology) , monoclonal antibody , glycoprotein , dermis , biophysics , chemistry , materials science , antibody , pathology , biology , medicine , immunology , biochemistry , physics , alternative medicine , optics
SUMMARY We report an ultrastructural study of the reactivity of an IgG monoclonal antibody, which reacts with human dermal elastic fibres. On electron microscopy, immunogold labelling demonstrated the reactivity of HB8 with the mirofibrillar component of the fibres. In contrast, the electrondense granular component which contains elastin was unlabelled with HB8. A fine network of microfibrils from the level of the oxytalan fibres to the large elastic fibres of the deep dermis was labelled with gold particles. No reactivity was detected on collagen fibres. These results demonstrate that HB8 reacts with the microfibrillar glycoproteins of the elastic fibres in normal skin. The precise biochemical nature of the antigens identified by HB8 is still unknown.