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Mononuclear cell subpopulations in the skin defined by monoclonal antibodies after HLA‐identical sibling marrow transplantation
Author(s) -
ATKINSON K.,
MUNRO V.,
VASAK E.,
BIGGS J.
Publication year - 1986
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1986.tb02793.x
Subject(s) - sibling , monoclonal antibody , immunology , human leukocyte antigen , transplantation , peripheral blood mononuclear cell , bone marrow transplantation , medicine , histocompatibility testing , antibody , biology , bone marrow , antigen , genetics , psychology , developmental psychology , in vitro
SUMMARY Mononuclear cell subpopulations present in the skin of 36 recipients of HLA‐identical sibling marrow transplants were defined by immunoperoxidase using a battery of monoclonal antibodies to cell surface differentiation antigens. The T 4 ‐positive (T 4 +) (helper‐inducer T cells), T8+ (cytotoxic‐suppressor T cells) and the T6+ (Langerhans cells) decreased in number early post transplant and returned towards normal numbers from day 42 onwards. There was no evidence that either the T4+ or the T8+ subset was involved in cell‐to‐cell contact damage in acute graft‐versus‐host disease (GVHD). The paucity of lymphoid cell infiltration of the epidermis in acute GVHD suggested the possibility of a soluble factor being responsible for basal layer damage. In patients with chronic GVHD there was no evidence of T4+ lymphocyte involvement, but T8+ lymphocytes were present in increased numbers, suggesting a role for the T8+ population in the skin lesions of chronic GVHD, or possibly a reflection of the pattern of T4+ and T8+ cell reconstitution in the blood post‐transplant. Finally, our study provided no evidence that B1+ (B cells), Leu 7+ (natural killer cells), OKM1+(histiocytes) or OKT10+ cells were involved in cell‐to‐cell contact damage in either acute or chronic GVHD.