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Cell surface marker studies in a patient with cutaneous multilobated T‐cell lymphoma
Author(s) -
FATTOROSSI A.,
MORETTI S.,
PALERMO A.,
SANTUCCI M.,
BONDI R.,
GIANNOTTI B.
Publication year - 1985
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1985.tb02383.x
Subject(s) - monoclonal antibody , antigen , biology , lymphoma , monoclonal , immunology , t lymphocyte , lymphocyte , antibody , pathology , microbiology and biotechnology , medicine
SUMMARY The phenotypic profile of atypical cells from a patient with cutaneous multilobated T‐cell lymphoma was investigated using a multiparameter approach including evaluation of membrane markers, cytochemistry, and functional activity. Retroviral sequence restriction analysis was also used to investigate the presence of human T‐cell leukaemia/lymphoma virus type I (HTLV‐I) in atypical cells infiltrating the skin and in otherwise normal peripheral blood lymphocytes. The atypical cells appeared to belong to the T‐lineage demonstrating OKT11 positivity, E‐rosette formation, tartrte‐sensitive acid phosphatase and β‐glucuronidase activity, and consistent negativity for cytoplasmic and/or surface monoclonal immunoglobulins. However, they failed to stain for other T‐lymphocyte‐associated antigens, such as those defined by OKT3, OKT4, OKT6, OKT8, OKT9, OKT10, Leu‐2a and Leu‐3a monoclonal antibodies, and did not express a definite α‐naphthyl‐acetate esterase pattern. Additional studies including phagocytosis tests and a series of monoclonal antibodies against phagocytic and natural killer cell associated antigens were all negative. No HTLV‐I related sequences were found in either the cells infiltrating the skin or in circulating lymphocytes. To our knowlege, in previously reported cases of cutaneous multilobated cell lymphoma a clear T‐lymphocyte phenotypic profile was demonstrated. Our present data indicate that this is not always necessarily the case. The peculair phenotype we found might represent a transitional state between different T‐cell subsets or an as yet unrecognized phenotype of a neoplastic T‐lymphocyte which lacks a normal counterpart.