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Insulin receptors in cultured human keratinocytes
Author(s) -
VERRANDO P.,
ORTONNE* J. P.
Publication year - 1984
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1984.tb15612.x
Subject(s) - receptor , insulin , acanthosis nigricans , insulin receptor , binding site , keratinocyte , endocrinology , hormone , cell , chemistry , insulin receptor substrate , cell culture , medicine , human skin , insulin resistance , biology , biochemistry , in vitro , genetics
SUMMARY Insulin binding to receptors was studied for the first time in cultured normal epidermal human keratinocytes. Binding was measured at 16°‐C in steady‐state conditions using 125 I‐labelled iodohormone and varying concentrations of unlabelled hormone. Insulin binding was compatible with either (i) a two‐site model‐a high affinity site (about 6000 sites per cell) and a low affinity site (about 88,000 sites per cell) site‐or (ii) one class of binding site with negatively co‐operative interactions, or (iii) both these models may operate. Insulin receptors (IR) have been extensively studied in various biological systems, but not in epidermal cells. However, insulin resistance with defects in IR may be associated with cutaneous abnormalities (acanthosis nigricans). This led us to study insulin binding on normal human keratinocytes in culture.

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