Premium
Effect of retinoic acid and low calcium conditions on surface glycoconjugates defined by differential lectin labelling in mouse epidermal cell culture
Author(s) -
ROBINSON JUNE K.,
FREINKEL RUTH K.,
GOTSCHALK R.
Publication year - 1984
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1984.tb07307.x
Subject(s) - glycoconjugate , retinoic acid , lectin , peanut agglutinin , fluorescein isothiocyanate , biochemistry , calcium , sialic acid , biology , cell culture , fucose , microbiology and biotechnology , chemistry , glycoprotein , physics , genetics , organic chemistry , quantum mechanics , fluorescence , gene
SUMMARY The appearance of cell surface glycoconjugates (detected by fluorescein‐isothiocyanate‐conjugated lectins and bullous pemphigoid antibody) was serially examined in mouse epidermal cell cultures treated with trans ‐retinoic acid and aromatic retinoic acid (etretinate) and in cultures maintained under low calcium conditions. The changes in lectin staining occurred in concert with the process of differentiation as assessed by cell morphology and colony growth characteristics, and they correlated with the patterns observed in whole mouse skin. The keratocyte cultures treated with retinoic acid showed delayed and reduced differentiation and stratification, and this was associated with markedly reduced binding of lectins specific for N ‐acetyl‐glucosamine and fucose. The low calcium concentration produced similar changes. Thus, the loss of surface glycoconjugates in the epidermal cell culture system was not specific for either retinoic acid or low calcium, but correlated with the degree of cell differentiation.