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A routine immuno‐electron microscopic technique for localizing an auto‐antibody on epidermal basement membrane
Author(s) -
PROST C.,
DUBERTRET L.,
FOSSE M.,
WECHSLER J.,
TOURAINE R.
Publication year - 1984
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1984.tb07305.x
Subject(s) - basement membrane , pathology , peroxidase , antibody , electron microscope , chemistry , medicine , immunology , optics , enzyme , physics , biochemistry
SUMMARY A new diagnostic technique is described, in which 0.7 mm thick slices of skin are freshly cut, throughly washed, slightly fixed and directly incubated in peroxidase‐labelled antibodies. This easy technique allows routine immuno‐electron microscopic diagnosis of subepidermal auto‐immune bullous diseases, with excellent morphological results.