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Sweat production in the isolated eccrine sweat gland of the rat
Author(s) -
MICHIAKMIKOLAJCZAK B.B.
Publication year - 1984
Publication title -
british journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.304
H-Index - 179
eISSN - 1365-2133
pISSN - 0007-0963
DOI - 10.1111/j.1365-2133.1984.tb04728.x
Subject(s) - sweat , sweat gland , eccrine sweat gland , carbachol , medicine , endocrinology , bicarbonate , chemistry , duct (anatomy) , atropine , methacholine , anatomy , stimulation , respiratory disease , lung
Summary A method has been developed to isolate a single rat eccrine sweat gland, together with the attached sweat duct, and to induce sweat secretion directly from the gland in vitro . Sweat production was induced using carbachol (2 × 10 −5 M to 3 × 10 −5 M) and methacholine (2 × 10 −5 M) in isolated planter sweat glands maintained in a suitable environment (modified Krebs‐Ringer bicarbonate at 38°C containing 30% w/v fresh rat serum). The output of sweat was measured every 5 minutes for 85 minutes and the maximal final volume obtained with any one gland was 23 nl. Sweat production ceased on adminstration of appropriate concentrations of atropine (1 × 10 −5 M to 5 × 10 −5 M), withdrawal of calcium ions from the bathing fluid, and reduction of the medium temperature to 4°C.